Field testing of volatile substances in olive groves

In three experimental olive groves, one in the inland, on the coast and on the island of Brač, selected volatile substances are tested in such a way that one selected volatile substance is placed in each delta trap in 4 repetitions, as stipulated in the project contract. In each olive grove, control delta traps with hexane were set up, as well as delta traps with pheromone for the olive moth in order to monitor the flight of the pest throughout the flight season of the same. These traps are changed every 7 days, rotated in each olive grove, and the results of the olive moth catch are read (Figure 1 – 7). In the other three selected olive groves, in which the attraction of the olive fly will be tested, yellow sticked traps with an olive fly pheromone were placed (Figures 8 and 9). Once a week, the flight is monitored and the results of the population are read, in order to set up an experiment with combined traps and selected volatile substances for the specified pest.

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Figure 9

Participation in a scientific symposium

“56. Croatian and 16th International Symposium of Agronomists” was held in Vodice from 5 to 10 September 2021. On Wednesday, October 8, as part of the section “Agroecology, ecological agriculture and environmental protection”, the the poster work “Olive volatiles – possible attractants of olive pests” by the group of authors Ana Matešković, Marijana Popović, Tonka Ninčević, Maja Jukić Špika, Maja Veršić Bratinčević, Marija Mandušić, Jakša Rošin and Elda Vitanović was presented. In this work the preliminary results of the project “New methods in olive pests controlling using plant volatiles” (KK.01.1.1.04.0002) were disseminated to audience.

Field testing of selected volatiles to attract the olive moth

An experiment with RAG (delta) traps was set up to test the attraction of selected volatiles to the olive moth (Prays oleae Bern.). Experiment is placed in one olive grove at each of the three research areas (inland, coast, island). Three selected volatiles are tested in such a way that one selected volatile is placed in each delta trap in 4 repetitions, as stipulated in the project Agreement. In each olive grove, control delta traps with hexane were also set up, as well as delta traps with pheromone for the olive moth in order to monitor the flight of that pest throughout the flight season of the same. These traps will be changed every 7 days, rotated in each olive grove and the results of the olive moth catch will be read weekly.

Setting of RAG (delta) traps for olive moth
Setting of RAG (delta) traps for olive moth
Setting of RAG (delta) traps for olive moth
Reading of olive moth catch results on RAG (delta) trap
Filling of selected volatiles into dispensers for RAG (delta) traps
Filling of selected volatiles into dispensers for RAG (delta) traps

Scientific paper published in the journal Molecules

Scientific paper “Essential oil volatile fingerprint differentiates Croatian cv. Oblica from other Olea europaea L. cultivars”, by the group of authors Marijana Popović, Maja Jukić Špika, Maja Veršić Bratinčević, Tonka Ninčević, Ana Matešković, Marija Mandušić, Jakša Rošin, Marija Nazlić, Valerija Dunkić and Elda Vitanović was accepted and published in the journal Molecules 2021, 26 (2), 3533. The paper is visible on the WoS platform. The mentioned scientific paper is the second scientific paper published within the LoVeFly project so far.

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Selection of olive groves for field research

Posted on 3. srpnja 2021. by Jaksa Rosin

Prema planiranim projektnim aktivnostima obavljen je odabir maslinika u kojima će se provoditi daljnja testiranja odabranih hlapivih tvari na privlačenje maslininog moljca maslinin moljac (Prays oleae Bern.) i maslinine muhe (Bactrocera oleae Rossi) . Odabir maslinika je obavljen na način da su prvo izdvojeni maslinici koji svojom veličinom odgovaraju traženim uvjetima. Detaljnijim proučavanjem zemljopisnih položaja maslinika i njihovih karakteristika napravljen je uži odabir. Nakon razgovora s vlasnicima maslinika te spoznajom o načinu korištenja njihovih maslinika odabrana su po dva maslinika za svako uzgojno podpodručje masline u Republici Hrvatskoj (zaobalje, priobalje, otoci); od kojih će se u jednom provoditi testiranje hlapivih tvari na privlačenje maslinog moljca, a u drugom na privlačenje maslinine muhe

According to the planned project activities, the selection of olive groves in which further tests of selected volatile substances to attract the olive moth Olive moth (Prays oleae Bern.) and olive fly (Bactrocera oleae Rossi) will be carried out was made. The selection of olive groves was done in such a way that the olive groves were first selected by its size that corresponded to the required conditions. A more detailed study of the geographical locations of olive groves and their characteristics made a shortlist. After talking with the owners of the olive groves and learning about the way of use of their olive groves, two olive groves were selected for each olive growing area in the Republic of Croatia (inland, coast, islands); of which in one case volatile substances will be tested to attract the olive moth and in the other to attract the olive fly.

Olive grove in Blizna Donja – inland area – olive moth
Olive grove in Blizna Gornja – inland area – olive fly
Olive grove in Kaštel Stari – coastal area – olive moth
Olive grove in Trogir – coastal area – olive fly
Olive grove in Supetar – island area – olive moth
Olive grove in Bol – island area – olive fly

Presentation of the LoVeFly project at the Science Festival 2021.

From 10 to 15 May 2021, the Science Festival was held in Split. As part of the activities entitled “Mediterranean Agriculture and Forestry – Research at the Institute for Adriatic Crops and Karst Reclamation”, the Institute’s staff held a virtual workshop on Friday, May 14 from 10 am to 11 am. At the workshop they presented several selected current results, achievements and current projects implemented in the field of agriculture and forestry at the Institute by broadcasting from the laboratories, greenhouses, experimental sites and plantations. Among other activities, Ana Matešković presented the project “New methods in olive pests controlling using plant volatiles”.

Lecture at the 23rd international event of olive growers and olive oil producers “Noćnjak 2021.”

Organized by the Cooperative Alliance of Dalmatia, the 23rd International event of olive growers and olive oil producers “Noćnjak 2021” was held from 27 to 30 April 2021, this year for epidemic reasons online for the first time.

More details on Noćnjak 2021 (in Croatian)

As part of the Noćnjak 2021, a scientific-professional meeting was held under the leadership of PhD Mirella Žanetić, associate on the LoVeFly project. As part of meeting, the PhD student, Ana Matešković, M.Sc., had a lecture in Croatian entitled “Mirisne tvari masline koje bi mogle biti odgovorne za privlačenje maslininog moljca i muhe” (in English: “Fragrant substances of olive that could be responsible for attracting olive moth and olive fly”) by the group of authors Ana Matešković, M.Sc.; PhD Marijana Popović; PhD Maja Jukić Špika; PhD Tonka Ninčević; MSc Jakša Rošin; Marija Mandušić, mag.ing.agr.; PhD Maja Veršić Bratinčević, PhD Mirella Žanetić and PhD Elda Vitanović. In presentation she showed the results obtained during the first year of research of the mentioned project to interested olive growers and olive oil producers.

Lecture “Fragrant substances of olive that could be responsible for attracting olive moth and olive fly” (in Croatian)

Public procurement of the Gas Chromatograph-Mass Spectrometer (GC-MS)

Based on the conducted Open Public Procurement Procedure of small value, published on September 21, 2020, the Gas Chromatograph-Mass Spectrometer (GC-MS) model GCMS-QP2020NX with AOC6000 autosampler and required accessories from Shimadzu d.o.o. was selected. According to the signed Public Procurement Agreement, GC-MS was delivered and installed in the Laboratory for Metabolomics of the Institute for Adriatic Crops and Karst Reclamation, and two associates on the project were educated on its use.

Lecture at the Institute for Adriatic Crops and Karst Rclamation

On Friday, April 9, the lecture “New methods in olive pests controlling using plant volatiles – LoVeFly” was held in the Institute for Adriatic Crops and Karst Reclamation. Project leader PhD Elda Vitanović introduced the work on the project and the obtained results of the first year of research to the participants. The lecture was attended by about 20 participants.

Determination of total phenols

The determination of total phenolic compounds of olive fruits harvested in three phases of fruit maturity (green, semi-matured and matured fruit) was done at the Institute for Adriatic Crops and Karst Reclamation. According to the planned project activities 27 samples of olive fruits of the cultivars Lastovka, Leccino and Frantoio were analyzed on the High-performance liquid chromatography (HPLC) device.