Scientific paper “Essential oil volatile fingerprint differentiates Croatian cv. Oblica from other Olea europaea L. cultivars”, by the group of authors Marijana Popović, Maja Jukić Špika, Maja Veršić Bratinčević, Tonka Ninčević, Ana Matešković, Marija Mandušić, Jakša Rošin, Marija Nazlić, Valerija Dunkić and Elda Vitanović was accepted and published in the journal Molecules 2021, 26 (2), 3533. The paper is visible on the WoS platform. The mentioned scientific paper is the second scientific paper published within the LoVeFly project so far.
Prema planiranim projektnim aktivnostima obavljen je odabir maslinika u kojima će se provoditi daljnja testiranja odabranih hlapivih tvari na privlačenje maslininog moljca maslinin moljac (Prays oleae Bern.) i maslinine muhe (Bactrocera oleae Rossi) . Odabir maslinika je obavljen na način da su prvo izdvojeni maslinici koji svojom veličinom odgovaraju traženim uvjetima. Detaljnijim proučavanjem zemljopisnih položaja maslinika i njihovih karakteristika napravljen je uži odabir. Nakon razgovora s vlasnicima maslinika te spoznajom o načinu korištenja njihovih maslinika odabrana su po dva maslinika za svako uzgojno podpodručje masline u Republici Hrvatskoj (zaobalje, priobalje, otoci); od kojih će se u jednom provoditi testiranje hlapivih tvari na privlačenje maslinog moljca, a u drugom na privlačenje maslinine muhe
According to the planned project activities, the selection of olive groves in which further tests of selected volatile substances to attract the olive moth Olive moth (Prays oleae Bern.) and olive fly (Bactrocera oleae Rossi) will be carried out was made. The selection of olive groves was done in such a way that the olive groves were first selected by its size that corresponded to the required conditions. A more detailed study of the geographical locations of olive groves and their characteristics made a shortlist. After talking with the owners of the olive groves and learning about the way of use of their olive groves, two olive groves were selected for each olive growing area in the Republic of Croatia (inland, coast, islands); of which in one case volatile substances will be tested to attract the olive moth and in the other to attract the olive fly.
From 10 to 15 May 2021, the Science Festival was held in Split. As part of the activities entitled “Mediterranean Agriculture and Forestry – Research at the Institute for Adriatic Crops and Karst Reclamation”, the Institute’s staff held a virtual workshop on Friday, May 14 from 10 am to 11 am. At the workshop they presented several selected current results, achievements and current projects implemented in the field of agriculture and forestry at the Institute by broadcasting from the laboratories, greenhouses, experimental sites and plantations. Among other activities, Ana Matešković presented the project “New methods in olive pests controlling using plant volatiles”.
Organized by the Cooperative Alliance of Dalmatia, the 23rd International event of olive growers and olive oil producers “Noćnjak 2021” was held from 27 to 30 April 2021, this year for epidemic reasons online for the first time.
As part of the Noćnjak 2021, a scientific-professional meeting was held under the leadership of PhD Mirella Žanetić, associate on the LoVeFly project. As part of meeting, the PhD student, Ana Matešković, M.Sc., had a lecture in Croatian entitled “Mirisne tvari masline koje bi mogle biti odgovorne za privlačenje maslininog moljca i muhe” (in English: “Fragrant substances of olive that could be responsible for attracting olive moth and olive fly”) by the group of authors Ana Matešković, M.Sc.; PhD Marijana Popović; PhD Maja Jukić Špika; PhD Tonka Ninčević; MSc Jakša Rošin; Marija Mandušić, mag.ing.agr.; PhD Maja Veršić Bratinčević, PhD Mirella Žanetić and PhD Elda Vitanović. In presentation she showed the results obtained during the first year of research of the mentioned project to interested olive growers and olive oil producers.
Based on the conducted Open Public Procurement Procedure of small value, published on September 21, 2020, the Gas Chromatograph-Mass Spectrometer (GC-MS) model GCMS-QP2020NX with AOC6000 autosampler and required accessories from Shimadzu d.o.o. was selected. According to the signed Public Procurement Agreement, GC-MS was delivered and installed in the Laboratory for Metabolomics of the Institute for Adriatic Crops and Karst Reclamation, and two associates on the project were educated on its use.
On Friday, April 9, the lecture “New methods in olive pests controlling using plant volatiles – LoVeFly” was held in the Institute for Adriatic Crops and Karst Reclamation. Project leader PhD Elda Vitanović introduced the work on the project and the obtained results of the first year of research to the participants. The lecture was attended by about 20 participants.
The determination of total phenolic compounds of olive fruits harvested in three phases of fruit maturity (green, semi-matured and matured fruit) was done at the Institute for Adriatic Crops and Karst Reclamation. According to the planned project activities 27 samples of olive fruits of the cultivars Lastovka, Leccino and Frantoio were analyzed on the High-performance liquid chromatography (HPLC) device.
The scientific paper of the group of authors was published on the Web of Science:
Vitanović, E.; Lopez, J.M.; Aldrich, J.R.; Jukić Špika, M.; Boundy-Mills, K.; Zalom, F.G. Yeasts Associated with the Olive Fruit Fly Bactrocera oleae (Rossi) (Diptera: Tephritidae) Lead to New Attractants. Agronomy 2020, 10, 1501.
https://doi.org/10.3390/agronomy10101501 (IF 2.603, Q1)
Paper is available on: https://www.mdpi.com/2073-4395/10/10/1501
Maja Veršić Bratinčević, PhD is employed as a postdoctoral researcher at the Department of Applied Sciences of the Institute for Adriatic Crops and Karst Reclamation.
She graduated at the Faculty of Chemical Technology, University of Split, majoring in Chemistry and Technology of Mediterranean Cultures, with her dissertation entitled “Glucosinolate degradation products and other volatile compounds in endemic plants of the family Brassicaceae: Aurinia Sinuata (L.) GRISEB. and Aurinia Leucedea (GUSS.) C. KOCH”. She defended her doctoral thesis entitled “Addictive substances in biological samples: determination and stability” in 2015 at the Faculty of Chemical Engineering and Technology, University of Zagreb, where she earned her PhD in natural sciences. In 2018, she was elected as a research associate in the interdisciplinary field of science (pharmacy and basic medical sciences). She gained work experience as a research novice in toxicology laboratory at the Clinical Hospital Centre Split, at the Department of Pathology, Cytology and Forensic Medicine, and at the Faculty of Medicine, University of Split, where she was employed until 2018 as a postdoctoral researcher.
As an author or co-author, she participated in the publication of eight scientific papers, several conference papers, and chapters of the textbook “Fundamentals of Forensic Toxicology”. She is a mentor or co-mentor on 13 graduate or final theses. She has participated in various scientific conferences, workshops and seminars for professional development.
The main scientific interest includes the analysis of samples using instrumental analysis techniques and primarily the analysis of samples using chromatographic techniques. The area of her research will include examining the profiles of volatile substances and their impact on attracting the most significant olive pests.
Hydrodistillation of dried leaves of selected olive varieties harvested in ripe fruit phase was performed in a Clevenger apparatus (for two hours and 30 minutes) in the laboratory of the Institute for Adriatic Crops and Karst from September 24 to September 27. The essential oils obtained by the hydrodistillation process were dried with anhydrous sodium sulfate. Samples of essential oils prepared in this way are stored in glass bottles at a temperature of 4°C. These essential oils will determine the profile of volatile substances, which could be responsible for attracting the olive fruit fly (Bactrocera oleae Rossi).